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ligase chain reaction

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  • Ligase chain reaction — The Ligase chain reaction (LCR) is a method of DNA amplification. While the better known PCR carries out the amplificiation by polymerizing nucleotides, this method instead amplifies the nucleic acid used as the probe. For each of the two DNA… …   Wikipedia

  • ligase chain reaction — a type of DNA amplification technique that uses DNA ligase to link adjacent pairs of synthetic oligonucleotides that have been hybridized to complementary regions on each strand of the double stranded target DNA, with the product used as a… …   Medical dictionary

  • ligase chain reaction — Abbreviation: LCR A technique for the detection and amplification of target DNA sequences. Two oligonucleotides are synthesized which between them are complementary to the entire target sequence, one to the 5 side and one to the 3 side. If the… …   Glossary of Biotechnology

  • Polymerase Chain Reaction — Die Polymerase Kettenreaktion (englisch Polymerase Chain Reaction, PCR) ist eine Methode, um die Erbsubstanz DNA in vitro zu vervielfältigen. Dazu wird ein Enzym verwendet, die DNA Polymerase. Der Begriff Kettenreaktion beschreibt in diesem… …   Deutsch Wikipedia

  • Ligase-Kettenreaktion — Die Ligase Kettenreaktion (englisch Ligase Chain Reaction, LCR) ist seit 1989 ein Nachweisverfahren für geringste Mengen von Erbmaterial (DNA). Sie funktioniert ähnlich wie die Polymerase Kettenreaktion, nur unter Verwendung eines anderen Enzyms… …   Deutsch Wikipedia

  • reaction — 1. The response of a muscle or other living tissue or organism to a stimulus. 2. The color change effected in litmus and certain other organic pigments by contact with substances such as acids or alkalies; also the property that such substances… …   Medical dictionary

  • Butyrate-CoA ligase — In enzymology, a butyrate CoA ligase (EC number|6.2.1.2) is an enzyme that catalyzes the chemical reaction:ATP + an acid + CoA ightleftharpoons AMP + diphosphate + an acyl CoAThe 3 substrates of this enzyme are ATP, acid, and CoA, whereas its 3… …   Wikipedia

  • Long-chain-fatty-acid-(acyl-carrier-protein) ligase — In enzymology, a long chain fatty acid [acyl carrier protein] ligase (EC number|6.2.1.20) is an enzyme that catalyzes the chemical reaction:ATP + an acid + [acyl carrier protein] ightleftharpoons AMP + diphosphate + acyl [acyl carrier protein]… …   Wikipedia

  • Long-chain-fatty-acid-luciferin-component ligase — In enzymology, a long chain fatty acid luciferin component ligase (EC number|6.2.1.19) is an enzyme that catalyzes the chemical reaction:ATP + an acid + protein ightleftharpoons AMP + diphosphate + an acyl protein thioesterThe 3 substrates of… …   Wikipedia

  • DNA ligase — DNA li·gase (liґgās) an enzyme of the ligase class that catalyzes the energy driven linkage of a double stranded DNA chain with a free 3′ hydroxyl group to one with a 5′ phosphate group, forming a phosphodiester bond between them …   Medical dictionary

  • Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… …   Wikipedia

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